pyrosequencing-based analysis of 16s rrna genes  (Pyrosequencing Inc)

Journal: Cureus

Article Title: Microbiota Dysbiosis a Cause of Colorectal Cancer or Not? A Systematic Review

doi: 10.7759/cureus.30893

Figure Lengend Snippet: Characteristics of papers included in the study rRNA- Ribosomal ribonucleic acid; CRC-Colorectal Cancer; DNA-Deoxy-ribonucleic acid; PCR- Polymerase Chain reaction; qPCR- Quantitative Polymerase Chain reaction; ETBF-Enterotoxigenic Bacteriodes fragilis ; RNA-seq-Ribosomal nucleic acid sequence; GA11x- Genome Analyzer 11x; F.nucleatum - Fusobacterium nucleatum ; CpG islands- Cytosine-Phosphate-Guanine Islands; rDNA-Ribosomal Deoxy-ribonucleic acid; IL17A- Interleukin 17A; 1L21- Interleukin 21; TNF- Tumour necrosis factor; CCL20- Chemokine ligand 20; P.anaerobius - Peptostreptooccus anaerobius ; ROS- Reactive Oxygen Species; TLR2-Toll-like receptor 2; TLR4- Toll-like receptor 4

Article Snippet: Gao et al. [ ] , 2015 , Narrative Review , Pyrosequencing-based analysis of 16S rRNA genes , , The study suggests gut dysbiosis is associated with CRC risk primarily through metabolic exchange or direct interaction with the host..

Techniques: Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Sequencing, Next-Generation Sequencing, Subculturing Assay, Immunofluorescence, Gene Expression, Construct, Infection, Methylation, In Vitro, Expressing, Bacteria

Journal: Frontiers in Microbiology

Article Title: Microbial Communities and Organic Matter Composition in Surface and Subsurface Sediments of the Helgoland Mud Area, North Sea

doi: 10.3389/fmicb.2015.01290

Figure Lengend Snippet: Composition of Bacterial (A) and Archaeal (B) communities in the Helgoland mud area based on pyrosequencing analyses of 16S rRNA gene data from core HE376-007-5 .

Article Snippet: To better understand relationships between molecular composition of a potentially bioavailable fraction of organic matter and microbial populations, bacterial and archaeal communities were characterized using pyrosequencing-based 16S rRNA gene analysis in surface (top 30 cm) and subsurface/deeper sediments (30–530 cm) of the Helgoland mud area, North Sea.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Microbial Communities and Organic Matter Composition in Surface and Subsurface Sediments of the Helgoland Mud Area, North Sea

doi: 10.3389/fmicb.2015.01290

Figure Lengend Snippet: Depth profiles of dominant Bacteria ( Chloroflexi ) and Archaea ( Thermoplasmata and MCG) in relation to TOC content at sediment depths from which DNA was extracted for pyrosequencing analysis of 16S rRNA genes . Gray bar represents SMT.

Article Snippet: To better understand relationships between molecular composition of a potentially bioavailable fraction of organic matter and microbial populations, bacterial and archaeal communities were characterized using pyrosequencing-based 16S rRNA gene analysis in surface (top 30 cm) and subsurface/deeper sediments (30–530 cm) of the Helgoland mud area, North Sea.

Techniques: Bacteria